Modeling tumor development and metastasis using paired organoids derived from patients with colorectal cancer liver metastases.

ABSTRACT: Tumor metastasis accounts for the majority of cancer-related deaths; it is therefore important to develop preclinical models that faithfully recapitulate disease progression. Here, we generated paired organoids derived from primary tumors and matched liver metastases in the same colorectal cancer (CRC) patients. Despite the fact that paired organoids exhibit comparable gene expression and cell morphology, organoids from metastatic lesions demonstrate more aggressive phenotypes, tumorigenesis, and metastatic capacity than those from primary lesions. Transcriptional analyses of the paired organoids reveal signature genes and pathways altered during the progression of CRC, including SOX2. Further study shows that inducible knockdown of SOX2 attenuated invasion, proliferation, and liver metast

SUBMITTER: Li H 

PROVIDER: S-EPMC7650218 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature