PRMT5 inhibition attenuates cartilage degradation by reducing MAPK and NF-κB signaling.

ABSTRACT:

Objectives

A role for the type II arginine methyltransferase PRMT5 in various human diseases has been identified. In this study, the potential mechanism underlying the involvement of PRMT5 in the pathological process leading to osteoarthritis (OA) was investigated.

Methods

PRMT5 expression in cartilage tissues from patients with OA and control individuals was assessed by immunohistochemical staining. The regulatory and functional roles of PRMT5 in the chondrocytes of patients with OA and control individuals were determined by western blotting and reverse transcription polymerase chain reaction. The effects of the PRMT5 inhibitor EPZ on interleukin-1β-induced inflammation were examined in the chondrocytes of patients with OA and in the destabilized medial meniscus (DMM) of a

SUBMITTER: Dong Y 

PROVIDER: S-EPMC7650297 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature