Identification of Candidate Genes Involved in Fruit Ripening and Crispness Retention Through Transcriptome Analyses of a 'Honeycrisp' Population.

ABSTRACT: Crispness retention is a postharvest trait that fruit of the 'Honeycrisp' apple and some of its progeny possess. To investigate the molecular mechanisms of crispness retention, progeny individuals derived from a 'Honeycrisp' × MN1764 population with fruit that either retain crispness (named "Retain"), lose crispness (named "Lose"), or that are not crisp at harvest (named "Non-crisp") were selected for transcriptomic comparisons. Differentially expressed genes (DEGs) were identified using RNA-Seq, and the expression levels of the DEGs were validated using nCounter®. Functional annotation of the DEGs revealed distinct ripening behaviors between fruit of the "Retain" and "Non-crisp" individuals, characterized by opposing expression patterns of auxin- and ethylene-related genes. However, both

SUBMITTER: Chang HY 

PROVIDER: S-EPMC7650588 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature