Single Cell Analysis of Drug Susceptibility of Mycobacterium Abscessus During Macrophage Infection.

ABSTRACT: Mycobacterium abscessus is an emerging health risk to immunocompromised individuals and to people with pre-existing pulmonary conditions. As M. abscessus possesses multiple mechanisms of drug resistance, treatments of M. abscessus are of poor efficacy. Therefore, there is an urgent need for new therapeutic strategies targeting M. abscessus. We describe an experimental system for screening of compounds for their antimicrobial activity against intracellular M. abscessus using flow cytometry and imaging flow cytometry. The assay allows simultaneous analysis of multiple parameters, such as proportion of infected host cells, bacterial load per host cell from the infected population, and host cell viability. We verified the suitability of this method using two antibiotics with known activity aga

SUBMITTER: Brzostek J 

PROVIDER: S-EPMC7650608 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature