3D-Printed Bubble-Free Perfusion Cartridge System for Live-Cell Imaging.

ABSTRACT: The advent of 3D-printing technologies has had a significant effect on the development of medical and biological devices. Perfusion chambers are widely used for live-cell imaging in cell biology research; however, air-bubble invasion is a pervasive problem in perfusion systems. Although 3D printing allows the rapid fabrication of millifluidic and microfluidic devices with high resolution, little has been reported on 3D-printed fluidic devices with bubble trapping systems. Herein, we present a 3D-printed millifluidic cartridge system with bent and flat tapered flow channels for preventing air-bubble invasion, irrespective of bubble volume and without the need for additional bubble-removing devices. This system realizes bubble-free perfusion with a user-friendly interface and no-time-penalty

SUBMITTER: Terutsuki D 

PROVIDER: S-EPMC7650622 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature