Unique and Specific m⁶A RNA Methylation in Mouse Embryonic and Postnatal Cerebral Cortices.

ABSTRACT: N⁶-methyladenosine (m⁶A)-mediated epitranscriptomic regulation is critical for various physiological processes. Genetic studies demonstrate that proper m⁶A-methylation is required for mouse brain development and function. Revealing landscapes of m⁶A-methylation in the cerebral cortex at different developmental stages will help to understand the biological meaning of epitranscriptomic regulation. Here, we depict the temporal-specific m⁶A-methylation status in mouse embryonic and postnatal cortices using methylated RNA immunoprecipitation (MeRIP) sequencing. We identified unique m⁶A binding motifs in stage-specific RNAs and found that more RNA transcripts are temporally methylated in embryonic cortices than in postnatal ones.

SUBMITTER: Zhang L 

PROVIDER: S-EPMC7650744 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature