Investigating hospital Mycobacterium chelonae infection using whole genome sequencing and hybrid assembly.

ABSTRACT: Mycobacterium chelonae is a rapidly growing nontuberculous mycobacterium that is a common cause of nosocomial infections. Here we describe investigation of a possible nosocomial transmission of M. chelonae at the Hospital of the University of Pennsylvania (HUP). M. chelonae strains with similar high-level antibiotic resistance patterns were isolated from two patients who developed post-operative infections at HUP in 2017, suggesting a possible point source infection. The isolates, along with other clinical isolates from other patients, were sequenced using the Illumina and Oxford Nanopore technologies. The resulting short and long reads were hybrid assembled into draft genomes. The genomes were compared by quantifying single nucleotide variants in the core genome and assessed using a contr

SUBMITTER: Gu CH 

PROVIDER: S-EPMC7652343 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature