Myocardial infarction affects Cx43 content of extracellular vesicles secreted by cardiomyocytes.

ABSTRACT: Ischemic heart disease has been associated with an impairment on intercellular communication mediated by both gap junctions and extracellular vesicles. We have previously shown that connexin 43 (Cx43), the main ventricular gap junction protein, assembles into channels at the extracellular vesicle surface, mediating the release of vesicle content into target cells. Here, using a comprehensive strategy that included cell-based approaches, animal models and human patients, we demonstrate that myocardial ischemia impairs the secretion of Cx43 into circulating, intracardiac and cardiomyocyte-derived vesicles. In addition, we show that ubiquitin signals Cx43 release in basal conditions but appears to be dispensable during ischemia, suggesting an interplay between ischemia-induced Cx43 degradatio

SUBMITTER: Martins-Marques T 

PROVIDER: S-EPMC7652393 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature