Deep Sequencing Identified Dysregulated Circulating MicroRNAs in Late Onset Preeclampsia.

ABSTRACT:

Background/aim

To characterize global microRNA (miRNA) expression profile in the first trimester maternal plasma of women who subsequently develop late-onset preeclampsia (LOPE) compared to uncomplicated pregnancies.

Materials and methods

Five first trimester plasma samples from women who developed LOPE and 5 controls were analyzed using next generation sequencing technology (NGS) followed by target prediction, Gene Ontology analysis and pathway identification. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed for confirmation in an independent cohort of 12 LOPE cases and 12 controls.

Results

miR-23b-5p and miR-99b-5p were down-regulated by >1.5 fold in LOPE complicated pregnancies (p value <0.05) compared to controls. Target prediction showed t

SUBMITTER: Mavreli D 

PROVIDER: S-EPMC7652460 | biostudies-literature | 2020 Sep-Oct

REPOSITORIES: biostudies-literature