Mitochondrial Ca^{2 +} Is Related to Mitochondrial Activity and Dynamic Events in Mouse Oocytes.

ABSTRACT: Mitochondrial energy insufficiency is strongly associated with oocyte activation disorders. Ca²⁺, especially that in the mitochondrial matrix, plays a pivotal role in mitochondrial energy supplementation, but the underlying mechanisms are still only poorly understood. An encoded mitochondrial matrix Ca²⁺ probe (Mt-GCaMP6s) was introduced to observe mitochondrial Ca²⁺ ([Ca²⁺]_m) dynamic changes during oocyte maturation and activation. We found that active mitochondria surrounding the nucleus showed a higher [Ca²⁺]_m than those distributed in the cortex during oocyte maturation. During oocyte partheno-activation, the patterns of Ca²⁺ dynamic changes were synchronous in the cytoplasm and mitochondria. Such h

SUBMITTER: Wang F 

PROVIDER: S-EPMC7652752 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature