Brassinosteroid Signaling Converges With Auxin-Mediated C3H17 to Regulate Xylem Formation in Populus.

ABSTRACT: Brassinosteroid (BR) signaling has long been reported to have an effect on xylem development, but the detailed mechanism remains unclear, especially in tree species. In this study, we find PdC3H17, which was demonstrated to mediate xylem formation driven by auxin in our previous report, is also involved in BR-promoted xylem development. Y1H analysis, EMSA, and transcription activation assay confirmed that PdC3H17 was directly targeted by PdBES1, which is a key transcriptional regulator in BR signaling. Tissue specificity expression analysis and in situ assay revealed that PdC3H17 had an overlapping expression profile with PdBES1. Hormone treatment examinations verified that xylem phenotypes in PdC3H17 transgenic plants, which were readily apparent in norm

SUBMITTER: Tang X 

PROVIDER: S-EPMC7652770 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature