Functional Characterization of a Dual Enhancer/Promoter Regulatory Element Leading Human CD69 Expression.

ABSTRACT: The CD69 gene encodes a C-type lectin glycoprotein with immune regulatory properties which is expressed on the cell surfaces of all activated hematopoietic cells. CD69 activation kinetics differ by developmental stage, cell linage and activating conditions, and these differences have been attributed to the participation of complex gene regulatory networks. An evolutionarily conserved regulatory element, CNS2, located 4kb upstream of the CD69 gene transcriptional start site, has been proposed as the major candidate governing the gene transcriptional activation program. To investigate the function of human CNS2, we studied the effect of its endogenous elimination via CRISPR-Cas9 on CD69 protein and mRNA expression levels in various immune cell lines. Even when the entire

SUBMITTER: Redondo-Anton J 

PROVIDER: S-EPMC7652794 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature