HIF-1α ameliorates tubular injury in diabetic nephropathy via HO-1-mediated control of mitochondrial dynamics.

ABSTRACT:

Objectives

In diabetic nephropathy (DN), hypoxia-inducible factor-1α (HIF-1α) activation in tubular cells plays an important protective role against kidney injury. The effects may occur via the target genes of HIF-1α, such as haem oxygenase-1 (HO-1), but the exact mechanisms are incompletely understood.

Materials and methods

Mice with proximal tubule-specific knockout of HIF-1α (PT-HIF-1α^-/- mice) were generated, and diabetes was induced in these mice by streptozotocin (STZ) injection. In addition, to mimic a hypoxic state, cobaltous chloride (CoCl₂ ) was applied to HK-2 cells.

Results

Our study first verified that conditional knockout of HIF-1α worsened tubular injury in DN; additionally, aggravated kidney dysfunction, renal histopathological al

SUBMITTER: Jiang N 

PROVIDER: S-EPMC7653251 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature