TGF-β/Smad2 signalling regulates enchondral bone formation of Gli1⁺ periosteal cells during fracture healing.

ABSTRACT:

Objectives

Most bone fracture heals through enchondral bone formation that relies on the involvement of periosteal progenitor cells. However, the identity of periosteal progenitor cells and the regulatory mechanism of their proliferation and differentiation remain unclear. The aim of this study was to investigate whether Gli1-Cre^ERT2 can identify a population of murine periosteal progenitor cells and the role of TGF-β signalling in periosteal progenitor cells on fracture healing.

Materials and methods

Double heterozygous Gli1-CreER^T2 ;Rosa26-tdTomato^flox/wt mice were sacrificed at different time points for tracing the fate of Gli1⁺ cells in both intact and fracture bone. Gli1-CreER^T2 -mediated Tgfbr2 knockout (Gli1-CreER

SUBMITTER: Xia C 

PROVIDER: S-EPMC7653269 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature