Slc39a5-mediated zinc homeostasis plays an essential role in venous angiogenesis in zebrafish.

ABSTRACT: Angiogenesis is a precise process mediated by a variety of signals and the environmental niche. Although the essential trace element zinc and its homeostasis are essential for maintaining proper cellular functions, whether zinc plays a role in angiogenesis is currently unknown. Using zebrafish embryos as a model system, we found that zinc treatment significantly increased the expression of the slc39a5 gene, which encodes the zinc transporter Slc39a5. Moreover, knocking down slc39a5 expression using either a morpholino or CRISPR/Cas9-mediated gene editing led to cardiac ischaemia and an accumulation of red blood cells in the caudal vein plexus (CVP), as well as delayed venous sprouting and fewer vascular loops in the CVP region during early development. Further analysis reveal

SUBMITTER: Xia Z 

PROVIDER: S-EPMC7653363 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature