Flt3L and GM-CSF enhance anti-tumor effect of HPV16/18 vaccine via increasing immune response.

ABSTRACT:

Objectives

Cervical cancer is the second leading cause of cancer death in women, which is closely related to persistent infection with high-risk Human papillomavirus (HPV). Therefore, it is important to develop new adjuvants for HPV vaccines. This research aimed to establish two new adjuvants which can enhance the immune effect of vaccines.

Materials and methods

C57BL/6 mice were divided into 5 groups and immunized by intramuscular injection of plasmid once every 2 weeks, three times in all. The growth and metastasis of tumors in mice was observed by in vivo imaging system (IVIS). Then, the mice were sacrificed and the pathological changes of organs were observed. In addition, the lymphocyte suspension was used for CLT killing test. IFN-γ level and the number of splen

SUBMITTER: Liu Y 

PROVIDER: S-EPMC7653621 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature