Improvement of stilbene production by mulberry Morus alba root culture via precursor feeding and co-elicitation.

ABSTRACT: Large amounts of Morus alba L. (MA) roots are needed as the source of active stilbenes in the industrial production of traditional medicines and cosmeceuticals. A recent investigation demonstrated resveratrol and its derivatives to be promising anti-COVID-19 agents. However, conventional cultivation of MA does not meet the demand for its stilbenes, and root quality usually varies between crops. This study established the in vitro non-GMO root culture of MA and optimized the root density, precursor feeding, and elicitors for stilbene productivity. A root culture with optimal inoculum density (3 g/flask of 30 mL medium) accumulated mulberroside A, oxyresveratrol, and resveratrol at 18.7?±?1.00 mg/g, 136?±?5.05 µg/g, and 41.6?±?5.84 µg/g dry weight (DW), respectively. The feeding of L-tyrosine shortened the time required to reach the stilbene productive stage. Root cultures co-treated with 200 µM methyl jasmonate and 2 mg/mL yeast extract accumulated the highest contents of mulberroside A (30.3?±?2.68 mg/g DW), oxyresveratrol (68.6?±?3.53 µg/g DW), and resveratrol (10.2?±?0.53 µg/g DW). In summary, root culture is a promising and sustainable source of stilbenes for the development of health products and agents for further investigation as potential anti-COVID-19 agents.

SUBMITTER: Inyai C 

PROVIDER: S-EPMC7653670 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature