Development of Styrene Maleic Acid Lipid Particles as a Tool for Studies of Phage-Host Interactions.

ABSTRACT: The infection of a bacterium by a phage starts with attachment to a receptor molecule on the host cell surface by the phage. Since receptor-phage interactions are crucial to successful infections, they are major determinants of phage host range and, by extension, of the broader effects that phages have on bacterial communities. Many receptor molecules, particularly membrane proteins, are difficult to isolate because their stability is supported by their native membrane environments. Styrene maleic acid lipid particles (SMALPs), a recent advance in membrane protein studies, are the result of membrane solubilizations by styrene maleic acid (SMA) copolymer chains. SMALPs thereby allow for isolation of membrane proteins while maintaining their native environment. Here, we explore SMALPs as a t

SUBMITTER: de Jonge PA 

PROVIDER: S-EPMC7654272 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature