A role for the Sts phosphatases in negatively regulating IFNγ-mediated production of nitric oxide in monocytes.

ABSTRACT:

Introduction

The atypical Sts phosphatases negatively regulate signaling pathways in diverse immune cell types, with two of their molecular targets being the related kinases Syk and Zap-70. Mice lacking Sts expression (Sts^-/- ) are resistant to infection by the live vaccine strain (LVS) of Francisella tularensis. Although the mechanisms underlying the enhanced resistance of Sts^-/- mice have not been definitively established, Sts^-/- bone marrow-derived monocytes (BMMs) demonstrate greater clearance of intracellular LVS following ex vivo infection, relative to wild type cells. To determine how the Sts proteins regulate monocyte bactericidal properties, we analyzed responses of infected cells.

Methods

Monocyte bacterial clearance was assayed usi

SUBMITTER: Parashar K 

PROVIDER: S-EPMC7654413 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature