Heterogeneity in human hippocampal CaMKII transcripts reveals allosteric hub-dependent regulation.

ABSTRACT: Calcium/calmodulin-dependent protein kinase II (CaMKII) plays a central role in Ca²⁺ signaling throughout the body. In the hippocampus, CaMKII is required for learning and memory. Vertebrate genomes encode four CaMKII homologs: CaMKIIα, CaMKIIβ, CaMKIIγ, and CaMKIIδ. All CaMKIIs consist of a kinase domain, a regulatory segment, a variable linker region, and a hub domain, which is responsible for oligomerization. The four proteins differ primarily in linker length and composition because of extensive alternative splicing. Here, we report the heterogeneity of CaMKII transcripts in three complex samples of human hippocampus using deep sequencing. We showed that hippocampal cells contain a diverse collection of over 70 CaMKII transcripts from all four CaMKII-encoding genes. We chara

SUBMITTER: Sloutsky R 

PROVIDER: S-EPMC7654443 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature