DNA methylation modification is associated with gonadal differentiation in Monopterus albus.

ABSTRACT:

Background

Both testis and ovary can be produced sequentially in an individual with the same genome when sex reversal occurs in the teleost Monopterus albus, and epigenetic modification is supposed to be involved in gonadal differentiation. However, DNA methylation regulation mechanism underlying the gonadal differentiation remains unclear.

Results

Here, we used liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) to simultaneously determine endogenous levels of both 5-methyl-2'-deoxycytidine (m⁵dC) and 5-hydroxymethyl-2'-deoxycytidine (hm⁵dC) during gonadal differentiation. Overall DNA methylation level was upregulated from ovary to testis via ovotestis. As a de novo methylase, dnmt3aa expression was also upregulated in

SUBMITTER: Wang X 

PROVIDER: S-EPMC7654577 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature