RNA-seq reveals the diverse effects of substrate stiffness on epidermal ovarian cancer cells.

ABSTRACT:

Background

Increasing evidence has confirmed that ovarian cancer is a mechanically responsive tumor both in vivo and in vitro. However, an understanding of the complete molecular mechanism involved in the response to substrate stiffness is lacking, as the associated transcriptome-wide effects have not been mapped. This limited understanding has restricted the identification of potential mechanically responsive targets in ovarian cancer.

Results

To address these limitations, we used a polyacrylamide hydrogel system with a tunable Young's modulus that broadly ranged from soft (1 kPa) to normal (6 kPa) and stiff (60 kPa) and investigated the effect of substrate rigidity on the morphology, spreading area, and cytoskeleton of SKOV-3 epidermal ovarian cancer (EOC) ce

SUBMITTER: Yang X 

PROVIDER: S-EPMC7655203 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature