The origins of binding specificity of a lanthanide ion binding peptide.

ABSTRACT: Lanthanide ions (Ln³⁺) show similar physicochemical properties in aqueous solutions, wherein they exist as + 3 cations and exhibit ionic radii differences of less than 0.26 Å. A flexible linear peptide lanthanide binding tag (LBT), which recognizes a series of 15 Ln³⁺, shows an interesting characteristic in binding specificity, i.e., binding affinity biphasically changes with an increase in the atomic number, and shows a greater than 60-fold affinity difference between the highest and lowest values. Herein, by combining experimental and computational investigations, we gain deep insight into the reaction mechanism underlying the specificity of LBT3, an LBT mutant, toward Ln³⁺. Our results clearly show that LBT3-Ln³⁺ binding can be divided into th

SUBMITTER: Hatanaka T 

PROVIDER: S-EPMC7656248 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature