Fate and functional roles of Prominin 1⁺ cells in liver injury and cancer.

ABSTRACT: Prominin 1 (PROM1) is one of a few clinically relevant progenitor markers in human alcoholic hepatitis (AH) and hepatocellular carcinoma (HCC), and mouse liver tumor initiating stem cell-like cells (TICs). However, the origin, fate and functions of PROM1⁺ cells in AH and HCC are unknown. Here we show by genetic lineage tracing that PROM1⁺ cells are derived in part from hepatocytes in AH and become tumor cells in mice with diethyl nitrosamine (DEN)-initiated, Western alcohol diet-promoted liver tumorigenesis. Our RNA sequencing analysis of mouse PROM1⁺ cells, reveals transcriptomic landscapes indicative of their identities as ductular reaction progenitors (DRPs) and TICs. Indeed, single-cell RNA sequencing reveals two subpopulations of Prom1⁺ Afp<

SUBMITTER: Wu R 

PROVIDER: S-EPMC7656457 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature