Obligatory role for PKCδ in PIP₂ -mediated activation of store-operated TRPC1 channels in vascular smooth muscle cells.

ABSTRACT:

Key points

In vascular smooth muscle cells (VSMCs), activation of Ca²⁺ -permeable store-operated channels (SOCs) composed of canonical transient receptor potential channel 1 (TRPC1) subunits mediates Ca²⁺ entry pathways that regulate contraction, proliferation and migration, which are processes associated with vascular disease. Activation of TRPC1-based SOCs requires protein kinase C (PKC) activity, which is proposed to phosphorylate TRPC1 proteins to promote channel opening by phosphatidylinositol 4,5-bisphosphate (PIP₂ ). We investigated the identity of the PKC isoform involved in activating TRPC1-based SOCs in rat mesenteric artery VSMCs. TRPC1-based SOCs were reduced by PKCδ inhibitors and knockdown of PKCδ expression. Store depletion induced in

SUBMITTER: Martin-Aragon Baudel MAS 

PROVIDER: S-EPMC7656825 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature