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Urine metabolomics of rats with chronic atrophic gastritis.

ABSTRACT:

Background/aim

To use liquid chromatography-mass spectrometry (LC-MS) to identify endogenous differential metabolites in the urine of rats with chronic atrophic gastritis (CAG).

Materials and methods

Methylnitronitrosoguanidine (MNNG) was used to produce a CAG model in Wistar rats, and HE staining was used to determine the pathological model. LC-MS was used to detect the differential metabolic profiles in rat urine. Diversified analysis was performed by the statistical method.

Results

Compared with the control group, the model group had 68 differential metabolites, 25 that were upregulated and 43 that were downregulated. The main metabolic pathways were D-glutamine and D-glutamic acid metabolism, histidine metabolism and purine metabolism.

Conclusion

By searching for differential metabolites and metabolic pathways in the urine of CAG rats, this study provides effective experimental data for the pathogenesis and clinical diagnosis of CAG.

SUBMITTER: Zu GX

PROVIDER: S-EPMC7657567 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Urine metabolomics of rats with chronic atrophic gastritis.

Zu Guo-Xiu GX Sun Qian-Qian QQ Chen Jian J Liu Xi-Jian XJ Sun Ke-Yun KY Zhang Liang-Kun LK Li Ling L Han Tao T Huang Hai-Liang HL

PloS one 20201111 11

<h4>Background/aim</h4>To use liquid chromatography-mass spectrometry (LC-MS) to identify endogenous differential metabolites in the urine of rats with chronic atrophic gastritis (CAG).<h4>Materials and methods</h4>Methylnitronitrosoguanidine (MNNG) was used to produce a CAG model in Wistar rats, and HE staining was used to determine the pathological model. LC-MS was used to detect the differential metabolic profiles in rat urine. Diversified analysis was performed by the statistical method.<h4> ...[more]

PMID: 33175875

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2021-02-02 | GSE153224 | GEO

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