High-Throughput Generation of Product Profiles for Arabinoxylan-Active Enzymes from Metagenomes.

ABSTRACT: Metagenomics is an exciting alternative to seek carbohydrate-active enzymes from a range of sources. Typically, metagenomics reveals dozens of putative catalysts that require functional characterization for further application in industrial processes. High-throughput screening methods compatible with adequate natural substrates are crucial for an accurate functional elucidation of substrate preferences. Based on DNA sequencer-aided fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) analysis of enzymatic-reaction products, we generated product profiles to consequently infer substrate cleavage positions, resulting in the generation of enzymatic-degradation maps. Product profiles were produced in high throughput for arabinoxylan (AX)-active enzymes belonging to the glycoside hydrola

SUBMITTER: Mauricio da Fonseca MJ 

PROVIDER: S-EPMC7657626 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature