Galaxy CLIP-Explorer: a web server for CLIP-Seq data analysis.

ABSTRACT:

Background

Post-transcriptional regulation via RNA-binding proteins plays a fundamental role in every organism, but the regulatory mechanisms lack important understanding. Nevertheless, they can be elucidated by cross-linking immunoprecipitation in combination with high-throughput sequencing (CLIP-Seq). CLIP-Seq answers questions about the functional role of an RNA-binding protein and its targets by determining binding sites on a nucleotide level and associated sequence and structural binding patterns. In recent years the amount of CLIP-Seq data skyrocketed, urging the need for an automatic data analysis that can deal with different experimental set-ups. However, noncanonical data, new protocols, and a huge variety of tools, especially for peak calling, made it difficult to define

SUBMITTER: Heyl F 

PROVIDER: S-EPMC7657819 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature