Microfluidic characterization of macromolecular liquid-liquid phase separation.

ABSTRACT: Liquid-liquid phase separation plays important roles in the compartmentalization of cells. Developing an understanding of how phase separation is encoded in biomacromolecules requires quantitative mapping of their phase behavior. Given that such experiments require large quantities of the biomolecule of interest, these efforts have been lagging behind the recent breadth of biological insights. Herein, we present a microfluidic phase chip that enables the measurement of saturation concentrations over at least three orders of magnitude for a broad spectrum of biomolecules and solution conditions. The phase chip consists of five units, each made of twenty individual sample chambers to allow the measurement of five sample conditions simultaneously. The analytes are slowly concentrated via evap

SUBMITTER: Bremer A 

PROVIDER: S-EPMC7658026 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature