Integrative genomics reveal a role for MCPIP1 in adipogenesis and adipocyte metabolism.

ABSTRACT: Obesity is considered a serious chronic disease, associated with an increased risk of developing cardiovascular diseases, non-alcoholic fatty liver disease and type 2 diabetes. Monocyte chemoattractant protein-1-induced protein-1 (MCPIP1) is an RNase decreasing stability of transcripts coding for inflammation-related proteins. In addition, MCPIP1 plays an important role in the regulation of adipogenesis in vitro by reducing the expression of key transcription factors, including C/EBPβ. To elucidate the role of MCPIP1 in adipocyte biology, we performed RNA-Seq and proteome analysis in 3T3-L1 adipocytes overexpressing wild-type (_WTMCPIP1) and the mutant form of MCPIP1 protein (_D141NMCPIP1). Our RNA-Seq analysis followed by confirmatory Q-RT-PCR revealed that elevated MC

SUBMITTER: Losko M 

PROVIDER: S-EPMC7658075 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature