Phases of decompensation during acute ischemia demonstrated in an ex vivo porcine bladder model.

ABSTRACT:

Background

The aim of this project was to develop an ex-vivo porcine bladder model to test the effects of increasing durations of acute ischemia on detrusor function.

Methods

Porcine bladders were perfused through bilateral vesical arteries at physiologic flow (4 mL/min) and filled through a urethral catheter. Intravesical pressures were continuously recorded using standard urodynamics equipment. Bladder contractions, with simulated voiding, were induced by arterial infusion of KCl at 250 mL. Total, passive, and active pressures were recorded for each contraction and data were normalized to the control fill. Bladders underwent the following perfusion protocol by adjusting the arterial flow rates: Equilibration (4 mL/min), control (4 mL/min), partial ischemia (2 mL/min), glob

SUBMITTER: Swavely NR 

PROVIDER: S-EPMC7658133 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature