EMC6 regulates acinar apoptosis via APAF1 in acute and chronic pancreatitis.

ABSTRACT: Treatment of acute pancreatitis (AP) and chronic pancreatitis (CP) remains problematic due to a lack of knowledge about disease-specific regulatory targets and mechanisms. The purpose of this study was to screen proteins related to endoplasmic reticulum (ER) stress and apoptosis pathways that may play a role in pancreatitis. Human pancreatic tissues including AP, CP, and healthy volunteers were collected during surgery. Humanized PRSS1 (protease serine 1) transgenic (PRSS1^Tg) mice were constructed and treated with caerulein to mimic the development of human AP and CP. Potential regulatory proteins in pancreatitis were identified by proteomic screen using pancreatic tissues of PRSS1^Tg AP mice. Adenoviral shRNA-mediated knockdown of identified proteins, followed by func

SUBMITTER: Tan JH 

PROVIDER: S-EPMC7658364 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature