Production and characterization of the ¹³ C/¹⁵ N single-labeled insecticidal protein Cry1Ab/Ac using recombinant Escherichia coli.

ABSTRACT: Synthetic Cry1Ab/Ac proteins expressed by genetically modified (GM) crops have a high potential to control insect pests without utilizing large amounts of chemical insecticides. Before these crops are used in agriculture, the environmental fate and interactions in the soil must be understood. Stable isotope-labeled Cry1Ab/Ac protein is a highly useful tool for collecting such data. We developed a protocol to produce ¹³ C/¹⁵ N single-labeled Cry proteins. The artificially synthesized gene Cry1Ab/Ac of Bt rice Huahui No. 1, which has been certified by the Chinese government to be safe for human consumption, was subcloned into pUC57, and the expression vector pET-28a-CryAb/Ac was constructed and transformed into Escherichia coli BL21 (DE3) competent cells. Next, 0.2 mM i

SUBMITTER: Wang Z 

PROVIDER: S-EPMC7658450 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature