Bacterial-like nitric oxide synthase in the haloalkaliphilic archaeon Natronomonas pharaonis.

ABSTRACT: Bacterial nitric oxide (NO) synthases (bNOS) play diverse and important roles in microbial physiology, stress resistance, and virulence. Although bacterial and mammalian NOS enzymes have been well-characterized, comparatively little is known about the prevalence and function of NOS enzymes in Archaea. Analysis of archaeal genomes revealed that highly conserved bNOS homologs were restricted to members of the Halobacteria. Of these, Natronomonas pharaonis NOS (npNOS) was chosen for further characterization. NO production was confirmed in heterologously expressed His-tagged npNOS by coupling nitrite production from N-hydroxy-L-arginine in an H₂O₂-supported reaction. Additionally, the nos gene was successfully targeted and disrupted to create a Nmn. pharaonis nos mutant b

SUBMITTER: Orsini SS 

PROVIDER: S-EPMC7658456 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature