Green fluorescent protein-based lactate and pyruvate indicators suitable for biochemical assays and live cell imaging.

ABSTRACT: Glycolysis is the metabolic pathway that converts glucose into pyruvate, whereas fermentation can then produce lactate from pyruvate. Here, we developed single fluorescent protein (FP)-based lactate and pyruvate indicators with low EC₅₀ for trace detection of metabolic molecules and live cell imaging and named them "Green Lindoblum" and "Green Pegassos," respectively. Green Lindoblum (EC₅₀ of 30 µM for lactate) and Green Pegassos (EC₅₀ of 70 µM for pyruvate) produced a 5.2- and 3.3-fold change in fluorescence intensity in response to lactate and pyruvate, respectively. Green Lindoblum measured lactate levels in mouse plasma, and Green Pegassos in combination with D-serine dehydratase successfully estimated D-serine levels released from mouse primary culture

SUBMITTER: Harada K 

PROVIDER: S-EPMC7659002 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature