Rational engineering of Kluyveromyces marxianus to create a chassis for the production of aromatic products.

ABSTRACT:

Background

The yeast Kluyveromyces marxianus offers unique potential for industrial biotechnology because of useful features like rapid growth, thermotolerance and a wide substrate range. As an emerging alternative platform, K. marxianus requires the development and validation of metabolic engineering strategies to best utilise its metabolism as a basis for bio-based production.

Results

To illustrate the synthetic biology strategies to be followed and showcase its potential, we describe a comprehensive approach to rationally engineer a metabolic pathway in K. marxianus. We use the phenylalanine biosynthetic pathway both as a prototype and because phenylalanine is a precursor for commercially valuable secondary metabolites. First, we modify and overexpress the pathway to be r

SUBMITTER: Rajkumar AS 

PROVIDER: S-EPMC7659061 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature