Multilineage differentiation potential of hematoendothelial progenitors derived from human induced pluripotent stem cells.

ABSTRACT:

Background

Human induced pluripotent stem cells (hiPSCs) offer a renewable source of cells for the generation of hematopoietic cells for cell-based therapy, disease modeling, and drug screening. However, current serum/feeder-free differentiation protocols rely on the use of various cytokines, which makes the process very costly or the generation of embryoid bodies (EBs), which are labor-intensive and can cause heterogeneity during differentiation. Here, we report a simple feeder and serum-free monolayer protocol for efficient generation of iPSC-derived multipotent hematoendothelial progenitors (HEPs), which can further differentiate into endothelial and hematopoietic cells including erythroid and T lineages.

Methods

Formation of HEPs from iPSCs was initiated by inhibition of

SUBMITTER: Netsrithong R 

PROVIDER: S-EPMC7659123 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature