Determination of novel reference genes for improving gene expression data normalization in selected canine reproductive tissues - a multistudy analysis.

ABSTRACT:

Background

Real time RT-PCR (qPCR) is a useful and powerful tool for quantitative measurement of gene expression. The proper choice of internal standards such as reference genes is crucial for correct data evaluation. In female dogs, as in other species, the reproductive tract is continuously undergoing hormonal and cycle stage-dependent morphological changes, which are associated with altered gene expression. However, there have been few attempts published so far targeted to the dog aimed at determining optimal reference genes for the reproductive organs. Most of these approaches relied on genes previously described in other species. Large-scale transcriptome-based experiments are promising tools for defining potential candidate reference genes, but were never considered in this c

SUBMITTER: Nowak M 

PROVIDER: S-EPMC7659137 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature