A comprehensive investigation of metagenome assembly by linked-read sequencing.

ABSTRACT:

Background

The human microbiota are complex systems with important roles in our physiological activities and diseases. Sequencing the microbial genomes in the microbiota can help in our interpretation of their activities. The vast majority of the microbes in the microbiota cannot be isolated for individual sequencing. Current metagenomics practices use short-read sequencing to simultaneously sequence a mixture of microbial genomes. However, these results are in ambiguity during genome assembly, leading to unsatisfactory microbial genome completeness and contig continuity. Linked-read sequencing is able to remove some of these ambiguities by attaching the same barcode to the reads from a long DNA fragment (10-100 kb), thus improving metagenome assembly. However, it is not clear how

SUBMITTER: Zhang L 

PROVIDER: S-EPMC7659138 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature