Protein-caged zinc porphyrin as a carbonic anhydrase mimic for carbon dioxide capture.

ABSTRACT: Zinc tetraphenylporphyrin (Zn-TPP) solubilized by GroEL protein cage was prepared as a supramolecular mimic of carbonic anhydrase (CA) for CO₂ capture. It is shown that the soluble Zn-TPP-GroEL complex can be formed easily by detergent dialysis. The Zn-TPP/GroEL binding ratio was found to increase with their dialysis ratio until reaching the maximum of about 30 porphyrins per protein cage. Moreover, the complex showed hydrase activity that catalyzes the CO₂ hydration in HCO₃^- and H⁺. It is further seen that the catalytic activity of Zn-TPP-GroEL was about one-half of that of a bovine CA at 25 °C. On the other hand, as the temperature was increased to 60 °C close to an industrial CO₂ absorption temperature, the natural enzyme

SUBMITTER: Chi H 

PROVIDER: S-EPMC7659338 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature