Highly Multiplexed Single-Cell In Situ RNA and DNA Analysis by Consecutive Hybridization.

ABSTRACT: The ability to comprehensively profile nucleic acids in individual cells in their natural spatial contexts is essential to advance our understanding of biology and medicine. Here, we report a novel method for spatial transcriptomics and genomics analysis. In this method, every nucleic acid molecule is detected as a fluorescent spot at its natural cellular location throughout the cycles of consecutive fluorescence in situ hybridization (C-FISH). In each C-FISH cycle, fluorescent oligonucleotide probes hybridize to the probes applied in the previous cycle, and also introduce the binding sites for the next cycle probes. With reiterative cycles of hybridization, imaging and photobleaching, the identities of the varied nucleic acids are determined by their unique color sequences. To demonstrate

SUBMITTER: Xiao L 

PROVIDER: S-EPMC7660199 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature