In Vivo Validation of Alternative FDXR Transcripts in Human Blood in Response to Ionizing Radiation.

ABSTRACT: Following cell stress such as ionising radiation (IR) exposure, multiple cellular pathways are activated. We recently demonstrated that ferredoxin reductase (FDXR) has a remarkable IR-induced transcriptional responsiveness in blood. Here, we provided a first comprehensive FDXR variant profile following DNA damage. First, specific quantitative real-time polymerase chain reaction (qPCR) primers were designed to establish dose-responses for eight curated FDXR variants, all up-regulated after IR in a dose-dependent manner. The potential role of gender on the expression of these variants was tested, and neither the variants response to IR nor the background level of expression was profoundly affected; moreover, in vitro induction of inflammation temporarily counteracted IR response early after

SUBMITTER: Cruz-Garcia L 

PROVIDER: S-EPMC7660203 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature