Resistance analysis of cherry rootstock 'CDR-1' (Prunus mahaleb) to crown gall disease.

ABSTRACT:

Background

Crown gall disease, caused by the pathogenic bacterium Agrobacterium tumefaciens, is responsible for extensive economic losses in orchards. Cherry rootstock 'CDR-1' (Prunus mahaleb) shows high resistance but the mechanism remains unclear. Here, we examined the morphology of pathogen-infected root neck surface, determined the activity of 10 defense-related enzymes and the content of salicylic acid (SA) and jasmonic acid (JA), and also applied transcriptome analysis, transient expression and transgenic verification to explore the crown gall resistance genes in 'CDR-1' plants.

Results

In our study, peroxidase increased in the first 10 days, while phenylalanine ammonialyase and lipoxygenase increased in the first 15 days post-infection. Four key enzymes in the AsA-GSH

SUBMITTER: Liang C 

PROVIDER: S-EPMC7661173 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature