A microfluidic platform for in situ investigation of biofilm formation and its treatment under controlled conditions.

ABSTRACT:

Background

Studying bacterial adhesion and early biofilm development is crucial for understanding the physiology of sessile bacteria and forms the basis for the development of novel antimicrobial biomaterials. Microfluidics technologies can be applied in such studies since they permit dynamic real-time analysis and a more precise control of relevant parameters compared to traditional static and flow chamber assays. In this work, we aimed to establish a microfluidic platform that permits real-time observation of bacterial adhesion and biofilm formation under precisely controlled homogeneous laminar flow conditions.

Results

Using Escherichia coli as the model bacterial strain, a microfluidic platform was developed to overcome several limitations of conventional microfluidics s

SUBMITTER: Straub H 

PROVIDER: S-EPMC7661213 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature