Single-cell RNA sequencing confirms IgG transcription and limited diversity of V_HDJ_H rearrangements in proximal tubular epithelial cells.

ABSTRACT: Increasing evidence has confirmed that immunoglobulins (Igs) can be expressed in non-B cells. Our previous work demonstrated that mesangial cells and podocytes express IgA and IgG, respectively. The aim of this work was to reveal whether proximal tubular epithelial cells (PTECs) express Igs. High-throughput single-cell RNA sequencing (scRNA-seq) detected Igs in a small number of PTECs, and then we combined nested PCR with Sanger sequencing to detect the transcripts and characterize the repertoires of Igs in PTECs. We sorted PTECs from the normal renal cortex of two patients with renal cancer by FACS and further confirmed their identify by LRP2 gene expression. Only the transcripts of the IgG heavy chain were successfully amplified in 91/111 single PTECs. We cloned and sequenced 469 V_H

SUBMITTER: Deng Z 

PROVIDER: S-EPMC7661700 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature