A quick and sensitive diagnostic tool for detection of Maize streak virus.

ABSTRACT: Maize streak virus disease (MSVD), caused by Maize streak virus (MSV; genus Mastrevirus), is one of the most severe and widespread viral diseases that adversely reduces maize yield and threatens food security in Africa. An effective control and management of MSVD requires robust and sensitive diagnostic tests capable of rapid detection of MSV. In this study, a loop-mediated isothermal amplification (LAMP) assay was designed for the specific detection of MSV. This test has shown to be highly specific and reproducible and able to detect MSV in as little as 10 fg/µl of purified genomic DNA obtained from a MSV-infected maize plant, a sensitivity 10⁵ times higher to that obtained with polymerase chain reaction (PCR) in current general use. The high degree of sequence identity between

SUBMITTER: Tembo M 

PROVIDER: S-EPMC7661706 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature