Antibody Fc Glycosylation Discriminates Between Latent and Active Tuberculosis.

ABSTRACT:

Background

Mycobacterium tuberculosis remains a global health problem and clinical management is complicated by difficulty in discriminating between latent infection and active disease. While M. tuberculosis-reactive antibody levels are heterogeneous, studies suggest that levels of IgG glycosylation differ between disease states. Here we extend this observation across antibody domains and M. tuberculosis specificities to define changes with the greatest resolving power.

Methods

Capillary electrophoretic glycan analysis was performed on bulk non-antigen-specific IgG, bulk Fc domain, bulk Fab domain, and purified protein derivative (PPD)- and Ag85A-specific IgG from subjects with latent (n = 10) and active (n = 20) tuberculosis. PPD-specific isotype/subclass, PPD-specific anti

SUBMITTER: Lu LL 

PROVIDER: S-EPMC7661770 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature