The effect of fluid shear stress in hydrogen sulphide production and cystathionine γ-lyase expression in human early endothelial progenitor cells.

ABSTRACT:

Background

Physiological fluid shear stress has been shown to have a beneficial impact on vascular homeostasis. Endothelial progenitor cells (EPCs) make a significant contribution to maintaining endothelial integrity. Therefore, we hypothesised that shear stress-induced endothelium protection plays a role in hydrogen sulphide (H₂S) production and up-regulation of cystathionine γ-lyase (CSE) expression in EPCs.

Methods

Human EPC-derived CSE activity was detected by colorimetric assay, and H₂S production was evaluated by membrane adsorption method. Cell proliferation, migration, and adhesion were assessed by MTT, Transwell, and endothelial cell-mediated adhesion assays, respectively. Real-time polymerase chain reaction (RT-PCR) was carried out to analyse

SUBMITTER: Hu Q 

PROVIDER: S-EPMC7661880 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature