Genetically modified endothelial progenitor cells with hNotch1.ICN overexpression display facilitated angiogenesis.

ABSTRACT:

Background

This study focused on hNotch1.ICN overexpression and investigated how it affects the biological behavior of endothelial progenitor cells (EPC) in vitro.

Methods

CCK 8 assay was used to evaluate overexpressed hNotch1.ICN to determine how to influence EPCs' survivability. The Annexin V/PI method was used to detect overexpressed hNotch1.ICN and to influence EPC apoptosis. A flow cytometry instrument was used to assess the overexpression of hNotch1.ICN and determine how to influence the EPC cell cycle. Transwell was used to investigate how overexpressed hNotch1.ICN EPCs migrate using their endothelial ability and adhesive ability with activated endothelial cells and angiogenesis ability. After lentivirus gene transfection, qPCR and Western blot were used to det

SUBMITTER: Guo P 

PROVIDER: S-EPMC7661891 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature