Endothelial Basement Membrane Laminins as an Environmental Cue in Monocyte Differentiation to Macrophages.

ABSTRACT: Monocyte differentiation to macrophages is triggered by migration across the endothelial barrier, which is constituted by both endothelial cells and their underlying basement membrane. We address here the role of the endothelial basement membrane laminins (laminins 411 and 511) in this monocyte to macrophage switch. Chimeric mice carrying CX3CR1-GFP bone marrow were employed to track CCL2-induced monocyte extravasation in a cremaster muscle model using intravital microscopy, revealing faster extravasation in mice lacking endothelial laminin 511 (Tek-cre::Lama5^-/- ) and slower extravasation in mice lacking laminin 411 (Lama4^-/- ). CX3CR1-GFP^low extravasating monocytes were found to have a higher motility at laminin 511 low sites and to preferen

SUBMITTER: Li L 

PROVIDER: S-EPMC7662115 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature